《植物生理学报》 2015, 51(8): 1248-1256

盐芥和拟南芥在不同胁迫下生理及ABA受体基因的响应

宋晓峰¹, 司晓娇¹, 沈晓艳¹, 魏海超², 孙伟¹, 冯献忠², 张慧^1,*, 王增兰^1,*

¹山东师范大学生命科学学院, 山东省逆境植物研究重点实验室, 济南250014; ²中国科学院东北地理与农业生态研究所, 长春130102

通信作者：张慧；E-mail: wangzenglan666@aliyun.com, laohanzhang@hotmail.com；Tel: 0531-86180764; 13176681535

摘　要：

本文以盐生植物盐芥和甜土植物拟南芥为实验对象, 利用生物信息学手段分析两者ABA受体的进化关系和保守结构域, 比较和研究了盐芥和拟南芥在400 mmol•L^-1甘露醇、200 mmol•L^-1 NaCl和50 μmol•L^-1 ABA处理下的根生长, 相对含水量和渗透压等生理指标的差异及ABA受体基因在胁迫条件下的表达变化。结果表明, 在盐和干旱胁迫条件下, 盐芥根的伸长率和侧根数目显著高于拟南芥, 表明前者抗逆性较强。ABA受体基因的表达在两者之间存在差异, PYL4、PYL5、PYL6、PYL7和PYL9在两者中表达变化趋势一致, PYR1、PYL1、PYL10和PYL13在盐芥和拟南芥中表达趋势相反, 结合生理指标推测两者在ABA信号途径中可能存在差异。

关键词：盐芥; 拟南芥; ABA受体; 盐胁迫; 干旱胁迫

收稿：2015-03-11   修定：2015-06-22

资助：山东省科技计划项目(2012GGB01136)和济南市高校自主创新项目(201303050)。

Responses of Physiological Mechanism and ABA Receptor Genes in Thellungiella halophila and Arabidopsis thaliana under Different Stress

SONG Xiao-Feng¹, SI Xiao-Jiao¹, SHEN Xiao-Yan¹, WEI Hai-Chao², SUN Wei¹, FENG Xian-Zhong², ZHANG Hui^1,*, WANG Zeng-Lan^1,*

¹Key Lab of Plant Stress Research of Shandong Province, College of Life Sciences, Shandong Normal University, Jinan 250014, China; ²Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun 130102, China

Corresponding author: ZHANG Hui; E-mail: wangzenglan666@aliyun.com, laohanzhang@hotmail.com; Tel: 0531-86180764; 13176681535

Abstract:

Thellungiella halophila and Arabidopsis thaliana were adopted as materials in this study. The evolutionary relationship and conserved domains of ABA receptors were analyzed by bioinformatics tools. We compared the differences between T. halophila and Arabidopsis in root growth, RWC, osmotic pressure and the expression of ABA receptor genes under 400 mmol•L^-1 mannitol, 200 mmol•L^-1 NaCl and 50 μmol•L-1 ABA stress conditions, respectively. The results showed that T. halophila had higher root elongation and more lateral roots under salt and drought stress treatment, which revealed that T. halophila was more tolerant to abiotic stresses than Arabidopsis. Under the same treatment, the expression pattern of ABA receptors showed the difference between these two species. The expression of PYL4, PYL5, PYL6, PYL7 and PYL9 showed the same trend, while the expression of PYR1, PYL1, PYL10 and PYL13 had the opposite trend, which indicated that there may be some differences in ABA signaling pathway between T. halophila and Arabidopsis.

Key words: Thellungiella halophila; Arabidopsis thaliana; ABA receptors; salt stress; drought stress